Technical Information

Access to BD Biosciences FACSAria Web site
(Contains information as well as video)

BD Biosciences Flourescence Spectrum Viewer
This applet will help you determine which fluorochomes to consider using for your sorting experiments. It takes a few seconds to load, so be patient. Under "Cytometer", use the pull down menu to select "FACSAria". You can toggle excitation and emission spectra on/off, or magnify the spectral area by dragging the cursor left to right. Double clicking on the spectra will return to normal magnification.

Link to Fluorochrome Table - Salk Flow Cytometry Facility
This is an excellent table of fluorochromes which contains excitation and emission maxima, as well as molecular weights and notes.

Exitation and Emission Spectra for BD Living Colors(TM)
Fluorescent Proteins
This site contains spectral characteristics of GFP and related proteins.

BD Living Colors FAQs - Multi-color Labeling
A useful site containing questions and answers concerning the use of BD fluorescent probes. Check out the links along the right panel.

Other Useful Technical Resources 

Examples in Cell Preparaton for FACSAria Sorting

Informational Brochure on BD FACSAria High Speed Cell Sorter

BD Biosciences Fluorochrome Reference Chart 

Introduction to Flow Cytometry Manual 

Technical Specifications Brochure